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75199

ANTIFUNGAL ACTIVITY OF PLANT EXTRACTS, ESSENTIAL OILS, AND MICROBIAL CULTURE FILTRATES AGAINST Botrytis cinerea In-vitro

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Last updated: 22 Jan 2023

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Abstract

The effect of using plant extracts and oils of plant origin on the colony growth of Botrytis cinerea, the causal agent of gray mold of fruits and vegetables was studied five plant extracts (pomegranate peel, cloves, neem, cinnamon and thyme) at three concentrations(0, 15, 30 and 45%; for the aqueous extracts and 0, 6, 9 and 15%; for the extracts of methyl alcohol and ethyl estate) were used Essential oils of colves, neem, cinnamon, and thyme at three concentrations (0, 1, 1,5 and 2%) as well as culture filtrates of Trichodermaharzianum, T. hamatum and Bacillus subtilis at three concentrations (0, 15, 30 and 45%) were also tested. The fungicide, Topsin M-70WP at the recommended concentration (80g/100L water) was used as a check for comparison.  In general, for the concentrations 1 and 2, there were no significant differences among the three methods of extraction (water, methyl alcohol, and ethyl acetate) also givenaqueous fraction of crude plant extracts significant reduction in the linear growth of B. cinerea ranged from (57.78 to 96.67%) Pomegranate peel andneem aqueous extracts caused the highest growth inhibition at 45% concentration 96.67 and 92.22% respectively with no significant difference from the fungicide effect at same concentration. While, given methyl alcohol and ethyl acetate All plant extracts produced significant growth inhibition of B. cinerea 7 days post inoculation in comparison with the untreated control. both fractions (methyl alcohol and ethyl acetate), it was found that all plant extracts tested at 15%, except thyme extract, caused more than 89% growth inhibition to the pathogen with no significant differences from the chemical fungicide, Topsin M-70WP. Essential oils given tested caused significant suppression of the pathogen growth, 7 days post inoculation in comparison with the untreated control. The growth inhibition of the pathogen increased with the increase of the plant oil concentration. The chemical fungicide, Topsin M-70WP, had the highest inhibitory effect against the pathogen's growth, which was followed by cloves and neem essential oils when tested at 2% concentration. While,all culture filtrates tested caused Record significant inhibition of the pathogen growth, 7 days after inoculation in comparison with the untreated control.. The bacterial culture filtrate of B. subtilis had the highest inhibitory effect against the pathogen's growth, with no significant difference from the chemical fungicide, Topsin M-70WP.  

DOI

10.21608/jppp.2015.75199

Keywords

Plant extracts, plant oils, microbial culture filtrate, Botrytis cinerea, biological control, antifungal activity, grape

Authors

First Name

Y.

Last Name

Shabana

MiddleName

M.

Affiliation

Plant Pathology Department, Faculty of Agriculture, Mansura University, Egypt

Email

yassershabana2@yahoo.com

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Orcid

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First Name

M.

Last Name

El-Boray

MiddleName

S.S.

Affiliation

Pomology Department, Faculty of Agriculture, Mansura University, Egypt

Email

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City

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Orcid

-

First Name

M.

Last Name

Mustafa

MiddleName

F.M.

Affiliation

Pomology Department, Faculty of Agriculture, Mansura University, Egypt

Email

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City

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Orcid

-

First Name

G.

Last Name

Al-Juboori

MiddleName

A.M.

Affiliation

Pomology Department, Faculty of Agriculture, Mansura University, Egypt Horticulture Department, Fac. Agric. Tech., SulaymaniaPolytechnic Univ., Iraq

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Volume

6

Article Issue

9

Related Issue

11313

Issue Date

2015-09-01

Receive Date

2020-03-03

Publish Date

2015-09-01

Page Start

1,297

Page End

1,311

Print ISSN

2090-3677

Online ISSN

2090-3758

Link

https://jppp.journals.ekb.eg/article_75199.html

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https://jppp.journals.ekb.eg/service?article_code=75199

Order

13

Type

Original Article

Type Code

888

Publication Type

Journal

Publication Title

Journal of Plant Protection and Pathology

Publication Link

https://jppp.journals.ekb.eg/

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Article

Created At

22 Jan 2023