Cotton breeders in Egypt pay a great attention to development hybrids has resistance  to several diseases and pests and give good yield. Pathogenicity tests of four Fusarium species ,namely: Fusarium poae; Fusarium oxysporum; Fusarium subglutinane and Fusarium solani (isolated from diseased cotton seedling). The diseases evaluation had spot line on the susceptibility of thirteen cultivars of Egyptian cotton (Gossypium barbadense L.), including Giza45, Giza70, Giza80, Giza81x83, Giza83, Giza84, Giza85, Giza86, Giza87, Giza88, Giza89, Giza86x89 and Giza90 under greenhouse conditions. The obtained  results revealed that at least every cultivar was resistant for one of  Fusarium  spices. Giza85 was the most susceptible cultivars (26.67; 40; 13.33 and 40 %, respectively) pre-emergence% for all tested Fusarium species. At the same time, Giza88 recorded resistance reaction ( 0.0; 6.67; 20 and 6.67% respectively). Fusarium oxysporum was the most pathogenic species (25.64% seedling death) followed by Fusarium solani and Fusarium poae (18.46 and 17.95%, respectively). The minimum incidence was noted in Fusarium subglutinane (9.74%), compared to 2.56% in check treat.