Effect of acetone extracts of grape branches, grape leaves, moringa and mulberry leaves on chitin deposition in mulberry silkworm larvae were studied. As well as the effect of those extracts on the characteristics of cocoons and silk threads were studied. Two concentrations were used (20 and 40%) of the extracts and sprayed on the mulberry leaves offered to larvae at the second day of fifth larval instar. Moringa leaves extract caused the most significant reduction in chitin deposition, especially after 120 h. While grape leaves extract gave increase in chitin deposition at all times in both tested concentration. In addition, the grape sticks extract gave the same effect in concentration 20% but, chitin reduction noticed in concentration 40% after 120 h. The results indicated that applying acetone extract of grape branches and moringa leaves at 20 % and grape leaf extract at 40 % to mulberry leaves recorded the highest fresh cocoon weight and cocoon shell weight. Grape leaves extract at 20 % and grape branches extract at 40 % increased the silk ratio. The highest length of silk threads obtained from silkworm larvae fed mulberry leaves treated with grape branches at 20 and 40 %. The acetone extracts of grape leaves, grape branches and mulberry leaves at concentration 20 % increased the silk filament weight and size. Conclusion: using acetone extracts of grape leaves and branches and moringa leaves in terms of juvenoids is a potent tool for improving silkworm performance and cocoon characters, which resulted in the production of high raw silk yield.