The present study was carried out to evaluate two naturalyte biocides, spinosad and ivermectin, one recommended chemical synthetic insecticide, malathion as reference against three important insects of stored grain, Sitophilus oryzae, Rhyzopertha dominica and Tribolium castaneum. Results obtained show that rates of spinosad or ivermectin from 50-150 ppm and rates of malathion from 1-4 ppm completely prevented emergence of adults of S.oryzae and followed that increase the reduction of progeny to 100%. R.dominica has the same trend with S.oryzae, but the R.dominica was more susceptible to biocides and insecticide tested than S.oryzae, which gave high mortality ranged from 81-100% at all concentrations. T.castaneum was more tolerant than the other tested insects. At the all rates of tested materials, the percentage loss weight of wheat decreased in comparison with control. In generally, R.dominica was the most susceptible to the tested materials followed by S.oryzae and T.castaneum . S.oryzae adults exposed to wheat grain diet received half-dose of 50 ppm spinosad or ivermectin, separately gave 96 and 88% mortality after two weeks of exposure, while the one-third dose gave 90 and 63% mortality. The high levels of both spinosad or ivermectin nearly completely prevented the F₁ progeny of  S.oryzae in half and one third dose. R.dominica was higher affected than S.oryzae at the same levels of spinosad and ivermectin where spinosad at the all levels of half dose completely prevented the F₁ progeny of R.dominica while with the one-third dose the % reduction values ranged from 82.3 to 100%.