Biological control of plant diseases especially root knot nematode has been handled in many scientific papers. Bacillus subtilis, Trichoderma harzianum (T1), T. viride (T2), mixture of both (T1andT2) in addition to the commercial product Nemastop were used under field conditions to control root knot nematode, Meloidogyne  javanica Chitwood on peanut plants which was the most frequent extracted from soil and roots  .  The efficacy of the treatments at different concentrations was also assayed under laboratory conditions as percentage of juvenile mortality and inhibition of egg hatching. All treatments revealed good effect in controlling root-knot at the highest concentrations, Nemastop was the most effective at(1:10), whereas, B. subtilis was the lowest one. Results obtained from field experiments were in harmony with those obtained from laboratory compared with control.  Efficacy of the treatment also positively correlated with number of application time. The plot treated with any one of the bioagents tested three times achieved highest effect in controlling root knot nematode and increased peanut yield. Under field condition mixture of Trichoderma isolates was the most effective followed by Nemastop.