The present investigation was carried outin the apiary yard at El Kharga Oases, New Valley Governorate, Egypt, during the period extended from January to December 2011. The results of the study reveal that The highest percentages of pollen collectors (28.12 and 14.3%) were recorded during May and September, respectively. The lowest percentages, 3.12 and 2.85%, were noticed throughout February and June, respectively. Maximum number of pollen collectors was noticed during the period from 11 a.m. to 1 p.m. (0.23 bees/ 2 min. / colony). Minimum number of pollen collectors was noticed during the period 7 - 9 a.m. (0.13 bees/ 2 min. /colony). The highest amount of pollen (810 gm. / colony) was collected during May, followed by September (412 gm. / colony). The lowest amount of pollen (82 gm. / colony) was collected during June, followed by February and December (90 and 93 gm. / colony), respectively.Significant correlation were detected between temperature daily range and the average amounts of pollen collection, where, r= 0.310.Highly significant correlation were detected between relative humidity (R.H. %) and the average amounts of pollen collection (gm./colony/ 2h.), where, correlation coefficient (r) = - 0.405.Species composition of collected pollen loads by honeybee workers appeared that, there are 20 different floral sources in El Kharga Oases.Such information will be useful for improving honeybee colony status and development in New Vally especially during the pollen shortage periods.