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53200

SILENCING THE EGYPTIAN COTTON LEAFWORM; Spodoptera littoralis (BIOSD.) CYTOCHROME P450 INHIBITS THE METABOLISM OF GOSSYPOL USING RNAI TECHNIQUE

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Last updated: 22 Jan 2023

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Abstract

RNA interference is an effective means regulate gene expression in both vitro and vivo. P450 monooxygenase plays a vital role in adaptation to cotton defence compounds such gossypol; toxic to Lepidopteran pests. This gene is highly expressed in insect midgut and its expression correlates with larval growth when gossypol is included in diet. Commonly, the transcription levels of P450 monoxygenase enzymes in the insect midgut are increased in the presence of gossypol compound. The current study is therefore revealed the inhibition effect of siRNA of cytP450 on the Egyptian cotton leafworm; Spodoptera littoralis (Biosd.)by selective targeting of cytochrome P450 gene. Small sequence of siRNA was designed and was subjected to cotton leafworm larvae using two different assays; diet mixing assay and force feeding assay. In diet mixing assay; the larval mortality percentage was ranged between 6.7 to 60% with 20 to 300ng siRNAi/g diet but this mortality showed 23.3 to 53.35 % in force feeding assay respectively 72h after treatment. Also, changes in esterase enzyme level was proved in insect gut (0.08 to 0.19 ng) in treated and untreated larvae when they subjected to diet incorporating 300ng/g siRNAi. Thus, the current investigation revealed that dsRNA used in this study plays as key factor for growth inhibition of cotton leafworm larvae which may consider as pave to develop transgenic cotton plants express siRNAi sequence that could reduce cottonleafworm population without insecticides intervention.

DOI

10.21608/jppp.2015.53200

Authors

First Name

S.

Last Name

Moussa

MiddleName

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Affiliation

Insect Biotechnology and Molecular Biology Unit, Plant Protection Research Institute, Agriculture Research Center, Dokki, Giza, Egypt www.insectbiotech.sci.eg

Email

saadmousa@insectbiotech.sci.eg

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Orcid

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First Name

Heba

Last Name

Abouelmaaty

MiddleName

G.

Affiliation

7 Nadi El-Seid St., Insect Molecular Biology and Biotechnology Unit, Plant Protection Research Institute, ARC, Dokki, Giza, Egypt

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Volume

6

Article Issue

1

Related Issue

8101

Issue Date

2015-01-01

Receive Date

2019-10-13

Publish Date

2015-01-01

Page Start

185

Page End

193

Print ISSN

2090-3677

Online ISSN

2090-3758

Article File

JPPP_Volume 6_Issue 1_Pages 185-193.pdf

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Link

https://jppp.journals.ekb.eg/article_53200.html

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https://jppp.journals.ekb.eg/service?article_code=53200

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18

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Original Article

Type Code

888

Publication Type

Journal

Publication Title

Journal of Plant Protection and Pathology

Publication Link

https://jppp.journals.ekb.eg/

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Article

Created At

22 Jan 2023