Two trials of winter seasons 2013 and 2014 were conducted in Lizemeter of Bacteriology Laboratory, Sakha Agricultural Research Station to investigate the potential effects of biofertilization and organic agriculture systems (had no mineral fertilizers nor chemical pesticides)compared to chemical fertilization on wheat grains yield (Egypt1 and Seds12 varieties) and susceptibility of the produced grains to infestation with Rhizopertha dominica post storage. Seds12 wheat variety positively responded to both organic agriculture, and bioinoculation treatments whereas grains yield significantly increased through both seasons compared to NPK fertilization (traditional control), but grain index did not exhibit any response. On the other hand, grain yield and grain index of Egypt 1 were not significantly affected. In respect to infestation with R. dominica during storage, according to the studied parameters i.e. mean number of adults emerged, mean developmental period (life cycle) and grain weight loss, Egypt1 variety negatively affected with significant values due to biofertilization treatment compared to NPK fertilization (control), while there were significant variations in case of organic agriculture treatment. On contrast, the grains of Seds12 variety exhibited significant decreases in mean adults emerged, grain weight loss and had the longest life cycle resultant biofertilization and organic agriculture treatments. So, it is considered moderate tolerant to R. dominica post harvest compared to traditional control