Eight bacterial isolatesbelonged to 4 genera, i.e., Serratia sp.,(one isolate) Bacillus subtilis, (three isolates) Bacillus thuringiensis,(two isolates) Streptomyces sp.(one isolate)and Pseudumonas flourescens(one isolate) and one fungal isolate (T. harzianum) were isolated from cucumber rhizosphere to evaluate their potential as antagonists to Sclerotiniastem and root rot on cucumber. In vitro, all isolates resulted in a significant reduction in hyphal growth of  pathogenic fungus, Streptomyces sp. was more significantly reduced  mycelium growth followed by P. flourescens and Bacillus subtilis (Bs1) (72.22, 68.0 and 62.22 %); respectively. All isolates gave a significant reduction in disease severity on cucumber plants, B2, Bs1, Bs2 and Streptomyces sp. isolates gave best reduction in disease severity. All isolates resulted in significant increase in morphological parameters of cucumber plants (stem and root length, foliage and root dry weight) compared to control.