This study was conducted to investigate the suppression effect of some antagonistic fungi against Rhizoctonia solani as the causal agent of basil root rot. Growth of R. solani was inhibited (in vitro and in vivo) in the presence of some antagonistic fungi(Trichoderma harzianum, Trichoderma hamatum, Trichoderma viride, Gliocladium virens, Gliocladium roseum). In vitro the results showed that T. harzianum was the best antagonistic fungi in inhibiting   the radial growth of R. solani gave (96.6%) inhibition, while G.roseum showed the lowest effect on R.solani (31.75%) inhibition compared with the untreated control .and the chemical control treatment that led to complete reduction in R. solani (100%) inhibition. In vivo experiments T. harzianum used by drench method (D) was the best antagonistic fungus had 0%disease severity followed by T.viride (D), T.hamatum (D)treatments had 7.41% disease severity. On the other hand, T.viride  used by soaked method (s) gave less effect on the pathogen with(40.74%) disease severity. Compared with the controls (0%) disease severity in the untreated control, also 55.56% in the artificially infested control and the chemical control it led that  22.22% disease severity, respectively).