This study was carried out during 2013 and 2014 seasons to investigate the effect of different biofertilizers (microbin, phosphorine and rhizobecteren) and weeds control treatments (flauzifop-p-butyl, bentazon, flauzifop-p-butyl + bentazon, hand hoeing twice and three times) on  roots nodulation, crop yield and associated weeds of soybean. The results showed that the bio-fertilizers and weeds control  treatments significantly decreased the dry weight of grassy weeds, broad-leaved and total weeds.  Also bio-fertilizers and weeds control  treatments increased  nodulation (number and  fresh weight nodules) and soybean growth and yield  characters (plant height, number of branches, number of pods, weight of pods, weight of seeds, number of seeds per plant as well as seeds yield ha^-1) in both growing seasons. Moreover, bio-fertilizers and weeds control  treatments increased seeds quality parameters (oil, protein and nutrients content) as well as enhanced nitrate reductase activity (NRA) in leaves and seeds of soybean in both growing seasons. Interaction between biofertlizers and weeds control treatments showed significant control of soybean weeds and increased crop yield of soybean.