The present work was carriedout at El-Arish, North Sinai Governorate, Egypt during two successive years (2014 and 2015) to study these points; Record the different plant species and their flowering periods as pollen sources of honeybees, evaluate pollen-collecting activity of the honeybee colonies, determine the chemical composition of collected pollen grains and identify the trapped pollen grains microscopically. The obtained results showed that, the plant species number recorded at El-Arish region as pollen sources of honeybees was 123 plants of 49 botanical families includes 4 groups, fruit and vegetables, ormental, wild weeds and medicinal plants. The most of the affiliated plants for 4 groups were flowering in the spring season. The results of pollen collection revealed that, Trapped pollens total weight in 1^st year of Italian race (1447 g/colony/year) was greater than those of Carniolan race (1415 g/colony/year). But, the total weight of trapped pollens in 2^nd year of Italian race (1350 g/colony/year) was lower than those of Carniolan race (1385 g/colony/year). The largest amounts were collected in April month. Chemical analysis of the major trapped pollen groups showed that pollen of palm recorded the highest level of moisture and crude lipids, while olive pollen recorded the highest amount of crude protein and ash. The highest crude fiber and educing sugar were recorded with orange pollen. The major trapped pollen groups were identified microscopically.