Experiments were conducted in the apiary ofHoney Bee ResearchCenter.A field experiment was carried out in 2014-2015at theExperimental Research Station of The Agriculture ResearchCenter, El-Arish, North Sinai,in order to study some factors affecting increase secretion ofhoney bee worker venom gland such as: The time factor for the extraction of dry bee venom by electrical impulses technique in practical experiments or on a commercial scale is important, where the extractive bee venom is higher after the sun set period in comparison to other day periods due to alsarah bees return to their colonies ,The position of venom collector device (VCD) also affects venom quantity, to put the bee venom collector between three hive positions (under frames, over frames &at the entrance). The best position for the bee venom collection was over the hive frames because guard bees sting the glass, and the allover board surrounding that exposed to the sting. This means increasing the exposed surface area of stings by bees also their effects on some biological activities of honeybee colonieswere studied.