This study were carried out to estimate theartificial supplements effect Prepared with brewer's yeast and yeast extracton amino acid composition of royal jelly (RJ) produced by Carniolianhybridhoneybee colonies. Considering these results, providing supplements for honeybee colonies subjected to royal jelly production can help and strengthen the technological development of the Egyptian beekeeping industry increasing its consumption in the national market. This research presents values analyzed of amino acid composition ofRJa little different from produced honeybee colonies fedon brewer's yeast and yeast extract. While found differences recorded between amino acids Serine (2.517&2.755mg/g.), and Threonine (1.441&1.629mg/g.) respectively, in RJ all tested samples. This fact shows the important to discuss or change the official method for royal jelly analysis. The characterization of physicochemical parameters is important in order to standardize quality royal jelly produced.