The present investigation studied bee venom collecting effect on the behavior of honeybee colonies such as (Hygienic and hoarding honeybees behavior, queen right and queen less). Hygienic behavior, the positive effect of using bee venom collection method on hygienic behavior, that increased this behavior (22%) of honey bee worker cells, Apis mellifera,while hoarding behavior in honey bees, Apis mellifera L., statistical analysis that there was no significant difference between before and after treatment in four colonies. on the other hand, there was an increase in feed conception after treatment, it that seems to the alarming or stimulation of worker honey bee by electrical impulses from bee venom collector so, it increasing the worker hoarding behavior. The queen less had negative effect on venom quantity, and there was positive relation between the number of combs and venom quantity.  The relationship between certain characters of honey bee colonies (i.e. stored pollen, stored honey yield and areas, bee population, brood, and foraging activities) and bee venom produced by electrical impulses and quantity of bee venom which collected from colonies variability  at different periods of active season showed significant variations in the venom amounts collected at different periods of active season.