According to soil isolationtechniques, 23 isolates of actinomycetes were obtainedfrom different soil samples in KSA and Egypt. All isolates were examined for their biological activity against both, the pestSpodopteralittoralis (Boisd.),also the plant pathogen Fusariumoxysporum. Results illustrated that, three isolates, named DI,DV and FII   exhibited high efficacy against the 4^th instar of S. littoralis, with mortality percentage 70, 80and 60%respectively. Also, other stages'development of S. littoraliswere affected. Meanwhile, metabolites of 20 isolates showed antagonistic activity against F.oxysporum, including the 3 isolates which showed efficacy against the insect pest, the % of inhibitionranged from67 – 49 %.These 3 isolates were belong  to the species StryptomyceslavendulaeandStr. clavuligerus.