An experiment was carried out at the farm of Sakha Agricultural Research Station, Kafr El-Sheikh governorate in 2014 and 2015 seasons. The objective was to study population fluctuations of the spiders and evaluation the efficacy of sampling methods for survey spiders at rice nursery. Specimens were collected from rice nursery by pitfall trap, water pan trap and sweep net were taken to the laboratory after labeling. All samples  were taken during April and the first week of May.  A total of 346 individuals belonging to 12 species and 8 families were recorded during the study period. The population density of family Tetragnathidae was found highest (28 individuals) followed by linyphiidae (20 individuals) in the first season. While in the second season the population density of family Linyphiidae was found highest (247 individuals) followed by Lycosidae (29 individuals). Indicated that  the water pan trap the highest efficacy  trap and trapped 51.47%  followed by pitfall trap 27.94% , meanwhile sweep net trap ranked the last category and represented by 20.58%  during the first season. While the pitfall trap the highest efficacy trap and trapped 45.87% followed by water pan trap40.26%, while sweep net trap came in the last category with 13.86% in the second season.