Field studies were carried out at the Experimental Farm, Faculty of Agriculture, Assiut University, during two successive seasons 2014 and 2015 in early summer plantation to study the influence of chemical and bio-fertilizers on, infestation of eggplant cultivars, black balady; white balady and romy with the two-spotted spider mite, Tetranychus urticae Koch, and some of eggplant cultivars characters; growth vegetative and fruit characters. Results indicated that T. urticae Koch started to appear with high mean numbers on eggplant cultivars in case of using chemical fertilizers (NPK) as compared with bio-fertilizers (Phosphorein and Nitrobein). The eggplant cultivars can be arranged according to the degree of infestation with T. urticae Koch as following white balady, black balady and romy, in either chemical or bio-fertilizers during the two seasons, 2014 and 2015. Also, using the bio-fertilizers increased significantly the growth vegetative (plant height and number of branches) and fruit characters (fruit length, fruit diameter and fruit weight) in the two seasons of study. According to mite infestation and the effect on the growth vegetative and fruit characters, bio-fertilizers may be recommend for use in eggplant varieties fertilization.