To investigate  the  protective effects of  biobran against N-nitrosodiethyamine (NDEA) and carbon tetrachloride CCl₄-induced hepatocarcinogenesis in rats. Hepatocarcinogenesis was induced in rats by a single intraperitoneal (i.p.) injection of N-nitrosodiethyamine (NDEA) at a dose of 200 mg/kg body weight followed by weekly subcutaneous injections of CCl₄ (3 ml/kg) for 6 weeks, as the promoter of carcinogenic effect. After administration of the carcinogen, 25 mg/kg/day of Biobran were administered i.p., five times a week throughout the study. At the end of  20 weeks, the body weight, liver weight were measured, blood samples  were collected for liver function tests, liver biopsies were processed for histopathology examination. Results demonstrated that biobran has significantly prevented the decrease of the body weight and the increase in the liver weight caused by NDEA.  Liver function tests showed significant increase in serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and γ -glutamyl transpeptidase (γ -GT) of untreated NDEA group, meanwhile treatment  with Biobran to rats exposed to carcinogens, significantly minimized the  elevation of  the  liver  function  enzymes level  to be comparable with the normal control values. Histopathological examination of the liver sections  of rats subjected to (DENA + CCl4) treatment revealed fibrosis and fatty infiltration of hepatocytes, with inflammatory collection and loss of architecture Biobran treatment showed minimal changes in hepatocyte morphology and histology with no inflammation. this study showed that  Biobran has a protective effect against hepatocarcinogenesis induced by NDEA and CCl4 in rats.