Tetranychus sp. is a globally spread economic pest that affects multiple plant species affecting the quality and the quantity of the crop. Because of its economic importance, the ecological and molecular perspective was investigated deeply for this pest. From the ecological perspective, the numbers and the relative infestation of Tetranychus were investigated for different cultivars of maize, and we had found that there wer three cultivars that were relatively resistant in Egypt; namely, Pioneer 3057, 321 and 352 under the prevailing conditions of temperature and humidity. From the molecular perspective, the DNA barcoding technique was applied for the first time in Egypt for Tetranychus species and its predators, and we had found two species of Tetranychus; namely, Tetranychus cinnabarinus and Tetranychus ludeni Zacher which would have been mistakenly identified as Tetranychus urticae Koch., in most of the cases due to the high resemblance in morphology,and also we had found a predator ;namely, Neoseiulus barkeri, as one of the predators of Tetranychus species. This research proved that combining both ecological and molecular analysis of the pest is very important for understanding the best protection and prevention options. Moreover, the molecular DNA barcoding is a very reliable tool in tetranychus species identification and its predators.