In a laboratory study investigated the changes in the metabolic products namely, total carbohydrates, total proteins and total lipids in the fourth larval instar of Spodopter alittoralis(Boisd.) tested with the LC₅₀ values. Compounds were one of plant growth regulator (Ethephon), emamectin benzoate (Highlex), chitin synthesis inhibitor (Novo), Bacillus thuringiensis( Protecto) and chlorpyrifos (Pestban) under laboratory conditions. Results indicated that, protecto showed the highest total carbohydrate content in the treated larvae (16.90 mg/g.b.wt.) than the other products followed by highlex, pestban, novo, and ethephon. The percentage of the change in the total carbohydrate content in the fourth larval instar treated with the LC₅₀ values recorded the highest decreasing percentage when treated larvae by plant growth regulator ethephon and represented by (-48.98%). Larvae exhibited the highest total protein content were treated with highlex (38.40mg/g.b.wt.) followed by pestban, protecto, novo then ethephon. Highest decreasing percentage of total protein content found in larvae treated with ethephon and represented by (-30.8%).Whereas the highest total lipids content was found in larvae treated with highlex (7.94 mg/g.b.wt.) followed by pestban, protecto, ethephon then novo.Highest decreasing percentage when treated larvae chitin synthesis inhibitor, (novo) and represented by (-18.60%).