Pest control has been achieved by chemical pesticides for more than 70 years but their efficiency is decreased as a result of insecticides resistant problems and environmental concern. The significant increase in pesticide use has increased concerns about potentially adverse effects on human health and the environment. Therefore, alternative insecticides with a new mode of action are needed. The present work aimed to evaluate the toxic effect of the insecticide, chlorantraniliprole (Coragen^® 20%SC) on liver and kidney biochemical parameters of albino rats with different sub-acute and sub-chronic doses_. The pathological parameters of sub-acute and sub-chronic liver, kidneys and protein profile changes by chlorantraniliprole were assessed. Generally, the tested compound caused a slight decrease in body weight as compared with untreated ones. Also, it caused an increase in AST and ALT activities urea and creatinine. Therefore, a decrease of albumin, globulin and blood calcium content was observed. The present results also clarify our need to avert exposure of humans to chlorantraniliprole and recommend that all pesticides no matter how safe they are must be assessed for their toxicity.