Honeybee is an important economic insect provide man with honey product give a good service by increasing crop production with his crop pollination services .Neonicotinoids  as Acetamprid ,Thianethoxam and Clothianidin introduced to the environment to control different types of pests attack cultivated crops.Oral and indirect contact trails were carried out on adult worker honey bees for each pesticide, using commercial formulations. The acute oral toxicity (LD₅₀)and the acute indirect contact toxicity ( LC₅₀) were calculated Mean LD₅₀ values and LC₅₀ values at 24 hrs for Acetamiprid was 114.72x10³ ng/bee , 1.58x10⁵ ppm , For Thiamethoxam was 740 ng/bee, o.15x10⁴ppm and for Clothianidin 330ng/bee, 8.8x10²ppm respectively. compared with traditional organophosphate Dimethoate  LD₅₀ 120 ng/bee ,and LC₅₀ 3.4x10⁴ppm . The data shows the same pattern after 48 and 72 hours of treatment. The neonicotinoids compounds are more safe to use against bees under laboratory condition at different time intervals compared with Dimethoate Organophoshate compound.. The safety margin for Acetamiprid LD₅₀ was 952, 917 and 984 after 24, 48 and 72 hours of application compared with dimethoate LD₅₀ , however due to LC₅₀ was 465, 836 and 592 times dimethoate toxicity. Acetamiprid was much safe more than Thiamethoxam than clothianidin.Neonicotinoids compound shows that its more safe to use in the environment while bees active or close to treated plants.