The genetic variation of the new Bacillus thuringiensis isolate from soil and (Agrin) as a reference were evaluated by using random amplified polymorphic (RAPD-PCR) in order to distinguish between the isolates using random primers. It was noticed that; 1- Genetically, B.t .isolate is the closest to Agrin which is a commercial product of B.t., 2- Estimating such genetic variations among and between different strains or isolates of B.t may be of practical value for designing integrated pest management (IPM) programs. It is hoped that the results obtained from this study may safely and effectively help in managing this serious insect pest of potato in an integrated pest management (IPM) program.