Carbohydrates, lipids and proteins contents were evaluated in 5^th larval instar haemolymph of Spodoptera littoralis (Boisd.) that treated with four isolates of entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, Paecilomyces lilacinus and Lecanicillium antillanum at 1^st, 2^nd, 3^rd and 4^th days. Generally, data in present work cleared that, there was significant decrease in haemolymph carbohydrates of larvae injected with the entomopathogenic fungi in the three time intervals (1^st, 2^ndand 3^rd) under investigation compared to control. The concentration level of protein content of larvae injected with the fungi significantly increased as compared with control insects in (1^st) day, then there is no significant change in the content after 2^nd and 3^rd day except increasing was noticed with the two fungi (B. bassiana and L. antillanum).There were significant increases of carbohydrates and protein contents observed in the last day (4^th day) due to effect of fungal infection. Significant increases of lipid content was noticed in the 5^thinstar cotton leaf worm larvae inoculated with the four tested fungi from 1^stto 4^th day. The overall results indicated that total S. littoralis larvae proteins, carbohydrates and lipids contents vary significantly during infection of the tested fungi at the four time intervals (1^st, 2^nd 3^rdand4^th days) and this tend to  dramatically changes in the composition of haemolymph, thus abnormal growth and development of insect caused.