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43886

Detection of Phytoplasmas Associated with Tomato Witches’ Broom and Big Bud Disease in Egypt

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Last updated: 04 Jan 2025

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Abstract

Symptoms of witches' broom were observed on tomato plants in some fields in Aswan governorate, Egypt. Molecular investigation was done with phytoplasma-detection primer P1/P7 to amplify a fragment of the 16S rRNA gene plus spacer region and beginning of 23Sr gene. RFLP analyses and sequencing of DNA fragments amplified by R16F2n/R2 primers indicated that these phytoplasmas are closely related and could be classified in the 16SrII group. Phylogenetic analyses of 41 accessions of 16S ribosomal RNA gene sequences of ‘Candidatus phytoplasmas' comprising the strain from Egypt and representative strains from GenBank confirmed that the phytoplasma from tomato cluster with other strains all classified in subgroup 16SrII-D. Therefore, it could be useful to use the RFLP methodology in rapid and specific screenings of this phytoplasma presence in Egyptian tomato plants

DOI

10.21608/jppp.2018.43886

Keywords

Tomato, phytoplasmas, 16SrII, RFLP

Authors

First Name

S.

Last Name

Behiry

MiddleName

I.

Affiliation

Agricultural Botany Department, Faculty of Agriculture Saba Basha, Alexandria University, Egypt.

Email

said.behiry@alexu.edu.eg

City

-

Orcid

-

Volume

9

Article Issue

9

Related Issue

6834

Issue Date

2018-09-01

Receive Date

2019-08-05

Publish Date

2018-09-01

Page Start

587

Page End

590

Print ISSN

2090-3677

Online ISSN

2090-3758

Article File

JPPP_Volume 9_Issue 9_Pages 587-590.pdf

PDF . 365.7kB

Link

https://jppp.journals.ekb.eg/article_43886.html

Detail API

https://jppp.journals.ekb.eg/service?article_code=43886

Order

7

Type

Original Article

Type Code

888

Publication Type

Journal

Publication Title

Journal of Plant Protection and Pathology

Publication Link

https://jppp.journals.ekb.eg/

MainTitle

Detection of Phytoplasmas Associated with Tomato Witches’ Broom and Big Bud Disease in Egypt

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Type

Article

Created At

22 Jan 2023