Pigeon droppings represent the most important source of pathogenic fungi of plants and other living organisms. The occurrence of fungi in samples of feral pigeon droppings was screened. Moreover, the antifungal properties of Artemisia herba-alba extract against these fungi was investigated. Minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of this extract were determined. The results revealed that Alternaria alternata recorded the highest frequency in the collected samples while, Cladosporium sphaerospermum was the least isolated fungus. Artemisia herba-alba extract showed the highest antifungal effect against C. sphaerospermum fungus by MIC and MFC values of 1.5% and 3%, respectively. However, A. alternata, Aspergillus niger, Fusarium oxysporum and Rhizopus stolonifer were the most resistant fungi to the extract by MIC value (6%). The highest MFC value (10%) was showed against A. niger, Mucor circinelloides, Penicillium citrinum and R. stolonifer fungal isolates.