Eight endophytic fungi were isolated from three Egyptian plants (Chenopodium album, Pelargonium graveolens and Malva parviflora). The fungi endophytes were identified by morphological and molecular methods as Fusarium chlamydosporum MG786540, Fusarium oxysporum MG786541, Alternaria alternata MG786542, Alternaria solani MG786543, Fusarium equiseti MG786544, Alternaria alternata MG786545, Stemphylium sp and Phoms sp. The endophytic fungi isolates exhibited antagonistic activities to phytopathogenic fungi by dual culture bioassay. A. alternata (MG786545) caused 32.58% and 44.68% growth inhibition of Fusarium oxysporum and Phytophthora infestance, respectively. F. equiseti (MG786544) caused 34.64% growth inhibition of Alternaria solani. Spore suspension of A. solani (MG786543), A. alternata (MG786545) and Stemphylium sp endophytes were tested for their herbicidal activity and plant growth promotion. A. solani (MG786543) at10⁸ spore/ ml inhibited 33.1% of seed germination and reduced 31.3%, 23.1% of shoot and root growth of Lolium temulentum, respectively. Stemphylium sp at10⁸ spore/ ml had the ability to enhance Triticum aestivum growth by increasing shoot and root lengths, and fresh and dry weights comparing to control.