Four antagonistic fungi, two antagonistic bacteria and three essential plant oils were used in vitro to control Fusarium oxysporum, F. solani and Sclerotium rolfsii causing rot root diseases in sugar beet. All antagonistic fungi showed variable antagonistic effect against sugar beet root rot pathogens. Result data showed that T. hamatum gave 76.3%,79.6% and 70.4% .T. harzianum giving 78.5%, 76.6% and 63.7% , T. viride gave 75.5%, 78.8% and 61.8%, while C. minitans gave 62.2%, 60% and 71.8% inhibition of F. oxysporum, F. solani and S. rolfsii respectively. Also all fungal culture filtrates gave highest effect against the pathogens at high concentration ranged between (76% to 93%) inhibition compared with untreated control. The antagonistic bacteria P. fluorescence recorded the best effect on mycelial growth of pathogens than B. subtilis. Where P. fluorescence inhibited growth of F. oxysporum, F. solani and S. rolfsii by 76%, 87.1% and 73.3% but B. subtilis gave 66.6%, 69.3% and 62.2% respectively. The culture filtrate of P. fluorescens exhibited high effect giving (79.3%, 82.6% and 75.5%) at 50% concentrations,  follwed by B. subtilis gave (72.2%, 64.8% and 68.8%) respectively with the same three pathogens compared with control. Cinnamon oils gave the highest antagonistic effect against all pathogens and completely inhibited growth (100%) at all concentrations with F. oxyporum and S. rolfsii, while with F. solani gave 93.2% inhibition at high concentrations, followed by spearmint oils which showed high effect with F. oxysporum and F. solani by (74.8% and 85.3% inhibition) at high concentrations, but gave moderate effect with S. rolfsii by 65.2% inhibition. Garlic oils exhibited high inhibition with S. rolfsii giving 95%, gave 71.5% and 76% with F. oxysporum and F. solani at same concentration compared with control.