The research was carried out at apiary of Department of Plant protection, Faculty of Agriculture, Al-Azhar University, Cairo, Egypt, to determine the effects of locality levels of hives on acceptance, mating success and pre-oviposition period of Carniolan F₁ hybrid and Italian F₁ hybrid queens and, the effect of locality levels of hives on brood inch² and pollen collection per gram, as well as, the effect on wasp traps efficiency under Nasr City conditions. the mean percentage of acceptance was 100% for lower and upper (Carniolan F₁ hybrid and Italian F₁ hybrid queens) except mating success in lower Carniolan F₁ hybrid was 88.89% while the pre-oviposition period for Carniolan F₁ hybrid and Italian F₁ hybrid were (12.50, 11 .83, 10.83 and 10.00 days) respectively. The mean of sealed worker brood inch square / colony were (133.33, 74.66, 46.60 and 35.11). The mean pollen gathering in gram / colony were (3.08, 4.33, 3.35, 2.76 and 2.35 for lower and 1.07, 1.55, 1.09, 0.81 and 1.00 for upper from (6-8, 8-10, 10-12, 12-2 and 2-4 Am) respectively. The relation between number of Vespa orientalis and pollen gathering was obviously noticed in upper more than lower. The results were statistically analyzed and discussed.