The present are pesiments wase condacted to study the biological aspects of the true spider Cheiracanthium isiacum O.P. under laboratory codeition . Individuals of Cheiracanthium isiacum O. Pickard-, 1874. Was collected from citrus trees in Aswan governorate, Egypt. it was fed and reared on adults of the two-spotted spider mite, Tetranychus urticae, vinegar fly, Dorsophila melanogaster and stored grain worm moth, Ephestia kuhniella. This species was found on citrus trees and on the ground and reared (during the period between 5 of February and 25 July 2016) .  Incubation period lasted on average of  17.0 days at 28 ± 2ºC and 60-70% R.H. There were 5 spiderling instars before reaching adult male and 6 instars before reaching adult female. The total period of spiderlings was shorter for male (57.3 days) than female (88.5 days). Adult longevity and life span were shorter for male than for the female. The pre- oviposition period was 9. 0 days while the oviposition period averaged 65.4, days and   the post- oviposition period lasted on   averag of 46.4 days.