The present study was carried out at the apiary of Plant Protection Department, Faculty of Agriculture, Al-Azhar University at Nasr city, Cairo Governorate, and Motobes region Kafr El-Sheikh Governorate. The study was designed to investigate the effect of the Carniolan and Italian hybrids and geographic regions on the honey bee venom production during different seasons, at the beginning from September 2017 to the end of August 2018.Data showed that the average amounts of dry bee venom collected from Carniolan and Italian colonies were 51.3 and 44.8 mg/colony, respectively, and there were no significant differences observed between dry bee venom amounts collected from the two honey bee hybrids.Results indicated that the highest amount of dry bee venom (99.9 mg/colony) was recorded in May, and the least amount (11.1 mg/colony) was recorded at October, under Nasr city conditions. While under Motobes region conditions, the highest amount of dry bee venom (102.5 mg/colony) was recorded in June, and the least amount of dry bee venom (60.2mg/colony) was obtained at the end of November.