Three entomopathogenic fungal secondary metabolites of Metarhizium anisopliae, Paceliomyces fumosoroseus and Trichoderma longibrachiatum were extracted using two different organic solvent and thoroughly investigated as bio-insecticide against nymphs and adults of onion thrips, Thrips tabaci using spraying method technique. The toxicity index of LC₅₀ values revealed that T. longibrachiatuom secondary metabolites extracts (chloroform and ethyl acetate) were the most effective against the two developmental stages (nymph stage and adult) of T. tabaci followed by M. anisopliae extracts and the least toxic one P. fuosoroseus extracts after 7-days of treatment. The chemical composition of the most promising extracts in each entomopathogenic fungi were analyzed using GC-MS technique. Thirty-five compounds belonging to six different classes of natural products were characterized.