The current experiment aims to study chemical analysis of the main pollen collection according to different regions At Kafr El-Sheikh Governorate (Sidi Salem, Bialla and Kafr El-Sheikh). The results showed that in the three districts during 2014 season, the highest average of moisture content was found in pollen obtained from Vicia fabae with 28.93%, 29.00%, and 29.00%, respectively mean while, the least values of moisture was found in Eucalyptus spp. in Sidi Salem, Bialla and Kafr El-Sheikh and represented by 27.70%, 28.50% and 26.90%, respectively. The highest ash content (4.44%) in Melilotus siculus in the three districts and the least average in Brassica Kabar (3.33%), the highest average of fiber content was found in Salix safsaf (2.31%) in the three districts and the least average was in Trifolium alexandrinum (1.27%), the highest average of lipid found in Salix safsaf (21.80%) and the least in Brassica kabar (20.73%), the highest average of protein found in Trifolium alexandrinum (32.17%) and the least average in Salix safsaf (30.78%) and the highest average of carbohydrate content was found in pollen Brassica kabar (14.22%) and the least average in Trifolium alexandrinum 12.67%.