Irrational utilization of chemical fertilization and/or pesticides in many field crops has resulted in some environmental hazardous as well as increasing production costs. Therefore, development of new cultivars responsive to low level of fertilization and simultaneously yield sustainable are very needed. However, the Egyptian National Wheat Res. Program has established a new breeding program for biofertilizer response. Six promising bread wheat lines were screened from N-biofertilization breeding program conducted at El-Bustan region. These lines sustained their yield even with the application of only 50% of the nitrogen recommended dose (286 kg/ha) when the biofertilizer “Azottin" was used. This result encouraged us to evaluate these lines under five nitrogen fertilizer levels (25, 50, 75, 100 and 125% of the recommended nitrogen dose using N-biofertilization in sandy soil at El-Bustan region, El-Behera Governorate during 1997/1998 and 1998/1999 wheat growing seasons. The results showed that the interaction between genotypes and nitrogen levels was significant for the yield and its components, while the harvest index was not significant. However, the highest grain yields were obtained from the biofertilized lines numbers 15, 11 and 21 at 214, kg N/ha, lines number 8 and 21 at 286 kg N/ha and lines 8 and 15 receiving 143 kg N/ha. Moreover, they surpassed the grain yields of the control treatment (286 kg N/ha) by 0.80, 0.87, 0.55, 0.72, 0.59, 0.52 and 0.30 ton/ha (14, 16, 10, 13, 11, 10 and 5%), respectively, in addition, these seven combinations exceeded the commercial cultivar Sids 1 under control treatment by 1.46, 1.23, 1.04, 1.16, 1.08, 0.96 and 0.96 ton/ha (29, 24, 21, 23, 21, 19 and 19%), respectively. The results indicated that lines; 8 and 15 expressed high stability level in grain yield over the two seasons. They also, outyielded the commercial check Sids 1, under biofertilization with no more than 143 kg N/ha, saving 50% of the recommended dose. Such lines should save money and environmental health. Therefore, the two lines are highly recommended for yield tests in the advanced yield trials, which conducted allover the country through the National Wheat Research Program.