The present investigation was carried out at Sakha Agricultural Research Station, Kafr El-Sheikh, Egypt, during the two growing seasons of 1997 and 1998. Two Egyptian cotton varieties, “Giza 45" and “Giza 76", belonging to Gossypium barbadense L. as well as their spontaneous offtypes, three offtypes for each, were used in this investigation. The principal components analysis indicated that the first four components accounted for more than 95% of the total variance over all studied traits, while the first principal component accounted for about 60% and 73% of all multivariate variation among genotypes for "Giza 45" and "Giza 76", respectively. Seed cotton yield/plant followed by lint index were the major contributer to genetic divergence among "Giza 45" genotypes, while lint colour, degree of yellowness and reflectance, contributed mostly to genetic divergence among "Giza 76" genotypes, reflecting the varied genetic architecture of both genotypes. The genetic divergence among the original parents and their offtypes based on Euclidean distances revealed the “naked seed-creamy lint" offtype, in both varieties, was wide divergent from their original parents and the other offtypes which were less divergent, exhibiting the largest dissimilarity coefficients. The dissimilarity coefficients between the reciprocal F₁ hybrids in both varieties, were not significant showing their close relationships and confirming the absence role of any maternal effects on genetic divergence. The naked-creamy offtype either in Giza 45 or in Giza 76 was widely distinct from its straight or reciprocal F₁ hybrids showing significant dissimilarity coefficients, such coefficients were more wide in Giza 76 rather than in Giza 45. The ten genotypes, four parents and six F₁ hybrids, were grouped into four clusters either among "Giza 45" or "Giza 76" genotypes based on the extent of their relative dissimilarities.