Isolation trials carried out on eggs, larvae, and adult females of root-knot nematodes extracted from root and rhizosphere soil samples resulted in the isolation of several fungal isolates. Screening of these isolates to verify their antagonistic activity against M. javanica resulted in the selection of four fungal isolates exhibiting marked antagonistic activity. They were identified as Myrothecium verrucaria, Acremonium sp < em>., Fusarium oxysporum and Geotrichum citri-aurantii. Results of in Situ experiments revealed that these four antagonistic fungi were able to invade and parasitize eggs of M. javanica in the presence of soil extract. However, they had different preferences to attack stages of nematode in water; Acremonium sp < em>. was most effective in reducing egg hatching, while, M. verrucaria was most effective against larvae. As far as we are aware, it is the first time that M. verrucaria was isolated from second stage larvae of root-knot nematode in Egypt. Pot experiments conducted to control root-knot disease in Vivo revealed that performance depends on application method, inoculum-density and antagonist. Some treatments achieved levels of protection against root-knot nematodes and of plant growth comparable to those achieved by the nematicide Furadan 10G. M. verrucaria  seemed to have some promising attributes, which increase its potential as a biocontrol agent In addition to its ability to achieve significant control levels against root-knot nematode, it can be easily mass produced as it grows abundantly on traditional media.