Effect of some chemicals (Pesticides) using various concentrations and some biological sources on radial growth of some soil- borne fungi (Pathogenic fungi) with two antagonistic fungi was studied The trail was done in vitro by growing infested agar discs on PDA containing  various concentrations of the chemical pesticides, i.e. fungicide, herbicide, nematicide and insecticide. The biological sources were antibiotics, and plant originated oils. The effects of three concentrations of each group were tested against six soil-borne fungal pathogens. The data obtained gave some attractive results, the fungicide vitavax 300and herbicide afalon  showed strong inhibitory effect on all pathogenic and antagonistic fungi in the three concentrations. The effects of the tested nematicide and insecticide were low when compared with the effect of the tested fungicide in the same trail, the lowest effect of the medium dose was obtained from Vydate on Verticillium albatrum with Trichoderma harzianum, the growth reduction percentages were (5.7-0.0); while the highest effect was obtained from Vydate on Pythium debaryanum with T.Legnorum (37.5-16.0). The medium dose of hostathion (insecticide) gave low inhibitory effect against Verticilliun alboatrum with T.Legnorum (6.7-0.0), while the highest effect was obtained from hostathion on Rhizoctionia solani with T.harzianum (40-20).The canestien gave very serious fungicidal effect against all treated soil- borne fungi with two antagonistic fungi, it was noticed that the reduction percentages using medium dose ranging between (75.0-92.0) was in the case of Pythium debaryanum and (88.9-92.6) in the case of R-solani with T.Legnorum ,( 73.3-91.10) in the case Pythium debaryanum and (88.6-92.9) in the case R.solani with T.harzianum.The tested antibiotic the chlorotetracyclin gave low inhibitory effect when compared with canestien. The plant originated oils treatments gave low and moderate effect in general, the highest effect was obtained from the garlic oil at low medium and high dose giving(61.5-42.0),(64.1-44.0) and (69.2-48.0) reduction in the radial growth percentages of R.solani with T.Legnorum, while the best results were obtained from mustard oil treatment giving reduction percentages of (37.5-10.0),(50.0-20)and(50.0-20.0)respectively with  P-debaryanum with T-legnorum.