There are two main methods for twist examination ,the first one is untwist method and the second is untwist-twist method . Giza 85 long staple (LS) and Giza 70 extra long staple (ELS) cotton varieties were spun to yarns of 30s,40s,50s and 60s counts and tested by the two methods based on recommended of (ASTM D 1422-85).One inch Distance between clamps and slight pretension were used in untwist method . In untwist -twist method distance between clamps of 3. 5. 10 and 15 inches were used . Pretensions of 2,3,4,5,6,7 and 8 grams were applied for each distance between clamps to select the appropriate distance and pretension. The results indicated that no significant differences between nominal twist and evaluated twist by the two methods . CV% within test specimens in untwist-twist method were more regular than that of untwist method. G70 was accomplished even yarns than G85 in the studied counts.. The appropriate distance between clamps in untwist-twist method was 15 inches for all tested counts. The appropriate pretensions were 8,5,4 and 4 grams for the counts 30s,40s,50s and 60s ,respectively.