Two field experiments were designed to study the effect of gibberellic acid (GA₃) at 0, 50, 100 and 200 mgl^-1 as pre-soaking of corms for 24 hr and bio-fertilizers [Nitrobine+Phosphorein (NP) 1:1 w/w] at rates 0.00  2.00 , 4.00 and 6.00 g/ plant). The aim of the present investigation was to evaluate the effect of gibberellic acid (GA₃)and bio-fertilization on growth, flowering and corm production of Gladiolus gandavensis L. cv. '' Rosesupreme ''. The obtained data showed that solo treating of gladiolus corms with GA_3, different rates of bio- fertilization and its combination stimulated the growth parameters  (plant height , number of leaves/ plant and leaves fresh weight / spike), flowering parameters (numbers of days from corms planting to flowering (day), number of florets /spike, and dry weights of spike L plant),leaves total chlorophyll , total carbohydratesof dry corms and yield production of corms. It can be recommended that treating gladiolus plants with GA₃ at200 mgL^-1 combined 4.00 and/or 6.00 g/ plant of bio-fertilizer (Nitrobine + Phosphorein 1:1 w/w) produce great yield of gladiolus plants (flowers and corms) with high quality