Protoplast fusion and conjugation were used to expand the Rhizobium host range. B.japonicum AS25 and R.leguminosarum biovar vicia ICARDA441 were fused and six, genetically stable, fusants were isolated. No fusants were obtained when B.japonicum BR20 and Agrobacterium tumefaciens CB were fused. Conjugation technique was done between Agrobacterium tumefaciens CB and twenty-five B.japonicum strains, which isolated from different Egyptian governorates. Fourteen conjugants were selected according to their variant antibiotic resistance (Genetic marker). The six fusants were able to nodulate both soybean and Vicia faba, while the fourteen conjugants were able to nodulate both clover and soybean. Plasmid profiles and phyllogenetic analysis of the parents; B.japonicum AS25 and R.leguminosarum biovar vicia ICARDA441,  and their six fusants were done. All fusants and conjugants were characterized and their relatedness were determined according to plasmid profiles.