257774

GENETIC IMPROVEMENT OF B. japonicum TO TOLERATE ABIOTIC STRESSES VIA PROTOPLAST FUSION

Article

Last updated: 04 Jan 2025

Subjects

-

Tags

-

Abstract

Two distinguished Bradyrhizobium japonicum Egyptian strains, i.e. SE8 and DO4, each was fused with the fast growing Rhizobium fredii USDA201 strain. Nine fusants were obtained in the first attempt (USDA 201 and SE8) and  four fusants were obtained in the second attempt ( USDA201 and DO4). All fusants were characterized on the bases of nodulation efficiency, salt and drought tolerance, heavy metals resistance and antibiotic resistance. Electrophoretic plasmid profiles of these fusants and their parents were studied and the relatedness between them was determined according to their phylogeneric tree. Some fusants retained their nodulation efficiency and showed higher efficiencies in salt, drought and /or heavy metals resistance than their parents.

DOI

10.21608/jpp.2001.257774

Keywords

Bradyrhizobium japonicum, Rhizobium fredii, genetic improvement, Intergeneric, protoplast fusion, Phylogenetic analysis

Authors

First Name

M.

Last Name

Abd El-Halim,

MiddleName

M.

Affiliation

Microbial Genetics Dept., National Research Center, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Abd El-Salam

MiddleName

S.

Affiliation

Microbial Genetics Dept., National Research Center, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

S.

Last Name

Ibrahim

MiddleName

A.

Affiliation

Genetics Dept., Faculty of Agriculture Ain Shams Univ., Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

S.

Last Name

Abd-El-Aal

MiddleName

Kh.

Affiliation

Microbial Genetics Dept., National Research Center, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

A.

Last Name

Attallah

MiddleName

G.

Affiliation

Microbial Genetics Dept., National Research Center, Cairo, Egypt

Email

-

City

-

Orcid

-

Volume

26

Article Issue

9

Related Issue

36488

Issue Date

2001-09-01

Receive Date

2001-08-08

Publish Date

2001-09-01

Page Start

5,427

Page End

5,436

Print ISSN

2090-3669

Online ISSN

2090-374X

Link

https://jpp.journals.ekb.eg/article_257774.html

Detail API

https://jpp.journals.ekb.eg/service?article_code=257774

Order

8

Type

Original Article

Type Code

887

Publication Type

Journal

Publication Title

Journal of Plant Production

Publication Link

https://jpp.journals.ekb.eg/

MainTitle

GENETIC IMPROVEMENT OF B. japonicum TO TOLERATE ABIOTIC STRESSES VIA PROTOPLAST FUSION

Details

Type

Article

Created At

22 Jan 2023