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257634

II- IN VITRO PROPAGATION OF DATE PALM (Phoenix dactylifera L.) cv. “ZAGHLOUL” BY SOMATIC EMBRYOGENESIS

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Last updated: 22 Jan 2023

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Abstract

The present investigation was carried out during three successive years (1999- 2001), in order to study the effect of the cytokinin (BA) to auxin (NAA) ratio in culture medium (Murashige and Skoog, 1962) on somatic embryogenesis in date  palm cv. “Zaghloul" cultures.   The main results can be summarized in the following points: 1-   Shoot tip explant proved to be the best in terms of callus formation percentage as compared with the leaf primordia explant. 2-   MS  (1962) medium supplemented with 3.0 mg1-1 2 ip + 30.0 mg1-1 NAA, the callus formation percentage was significantly the highest (100% in shoot tip explant and 50.0% in leaf primordia explant). 3-   Embryogenesis percentage was significantly the highest (87.5%) on MS (1962) medium supplemented with 3.0 mg1-1 B A + 1.0 mg1-1 NAA. On the same medium, average number of germinated embyoids per callus was significantly the highest (2.75). 4-   MS medium supplemented with 3.0 mg1-1  NAA in medium produced the highest rooting percentage (100 %), greatest average roots number per shoot (12.0) and the longest roots (10.0 cm). 5-   Finally, 70% of the obtained plants of date palm cv. “Zaghloul" were successfully transplanted to soil.

DOI

10.21608/jpp.2002.257634

Authors

First Name

M.

Last Name

El- Sabrout,

MiddleName

B.

Affiliation

Pomology Department, Faculty of Agriculture, Alexandria University, Alexandria, Egypt.

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Volume

27

Article Issue

12

Related Issue

36422

Issue Date

2002-12-01

Receive Date

2002-11-29

Publish Date

2002-12-01

Page Start

8,621

Page End

8,631

Print ISSN

2090-3669

Online ISSN

2090-374X

Link

https://jpp.journals.ekb.eg/article_257634.html

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https://jpp.journals.ekb.eg/service?article_code=257634

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29

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Original Article

Type Code

887

Publication Type

Journal

Publication Title

Journal of Plant Production

Publication Link

https://jpp.journals.ekb.eg/

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Article

Created At

22 Jan 2023