In this study, hair and nails levels of iron (Fe) and zinc (Zn) were determined in healthy adult women (No. 20) ages 18-21 year. The hair and nails contain high concentrations of zinc 15.39 ± 0.64; 14.48 ± 0.31 followed by iron 4.73 ± 0.16; 14.23 ± 0.43 respectively. The level of Fe and Zn were found to be lower than that reported at different places in the world. Many genera of microorganisms were isolated from the tested samples. Hair and nails can be considered as useful parameters for measurement of the accumulation of trace elements in the human body.