Two field experiments were conducted in saline sandy silt soils at Tag El-Eiz, Agricultural Research Station, Dakahlia Governorate, during the two successive growing seasons. 1999/2000 and 2000/2001. The objective of this work was to study the response of three wheat varieties namely Gemmeiza 7, Sakha 69 and Sakha 8 to inoculation with serealen (Bacteria Bacillus polymyxa) as a biofertilizer under four nitrogen levels used in this study, 20, 40, 60 and 80 kg The results indicated that wheat variety Gemmeiza7 gave the higest value of plant height, spike length, number of spikelets/ spike, number of grains/ spike, grains weight/ spike, 1000-grain weight, grain yield, straw yield and protein percentage, whereas Sakha 8 gave the highest value of number of spikes/ m2 Increasing nitrogen fertilizer levels from 20 to 80 kg N/ fad significantly increased plant height, number of spikes/ m2, spike length, number of spikelets/ spike, number of grains/ spike, grain weight/ spike, grain yield (ardab/ fad), straw yield (ton/ fad) and grain protein in both seasons. But application of 40 kg N/ fad in the first season and 20 kg N/ fad in the second season gave the highest value of 1000-grain weight.Wheat plants inoculated with serealin showed a significant increase for all studied treaits except for grain weight/ spike in the second season,1000-grain weight, grain yield (ardab/ fad), straw yield (ton/ fad) and protein percentage in both seasons.The results showed that the interaction between wheat varieties and biofertilizer was significantly affected the number of grains/ spike in the first season and spike length (cm), number of grains/ spike and grains weight (g) in the second season. On the other hand ,the interaction between nitrogen fertilizer and biofertilizer had a significant influence on plant height and grain weight/ spick (g) in the second season.