The preliminary phytochemical screening of Verbascum sinaticum which
collected from Catherin and Wadi Fieran showed that it contained sterols, flavonoids,
chlorides, tannins, resine, saponines, alkaloids, and carbohydrates and/or glycosides. The paper chromatography of free sugars showed that Verbascum sinaticum
contained glucose, fructose, rhamnose and arabinose in leave at the two studied
habitats, while it was obvious that rhamnose was absent in stem at Catherin and
rhamnose and fructose were absent In stem at Wadi Fleran.Conceming the combined
sugars glucose, rhamnose, sucrose and arabinose were detected in the leaves, while
the stem contained the same components except rhamnose at Catherin habitat. On
the other hand at Wadi Fieran habitat the results revealed the presence of glucose,
fructose, rhamnose, sucrose and arabinose in leave, while the stem contained the
same components except fructose and rhamnose. Investigation of free amino acid showed the present of proline, alanine and
lysine in leave and stem at the two studied habitats, while aspartic acid, serine,
glycine, valine, isoleucine and leucine present only in the stem at the two habitats but
varied in leave at the two habitats. Concerning the protein-amino acids, Verbascum
sinaticum contained seventeen amino acid with different concentrations. Proline was
the highest one of the bound amino acids 25.1 and 18.3% in leave at Catherin and
Wadi Fieran sample respectively. Meanwhile,leucine was the highest one 18.6 and
15.1% in the stem at the same habitats, respectively. The values of the fundamental chemical properties of the extracted lipid of
Vemascum sinsticum were lower in leaves than that found in stems, while acid,
iodine, ester and saponification values were higher at Wadi Fieran habitat than that
sample at Catherin habitat. The unsaponifiables content of Verbascum sinaticum was determined and
the relative percentages of each component were calculated. There was no significant
. difference between the two habitats except the presence of tetracosanoic at Catherin
only and the presence of cholesterol in leave and stem at catherin and Wadi Fieran
respectively. The Gas-liqued chromatographical analysis of Verbascum sinaticum lipids
revealed the presence of long chain fatty acid, where stearic acid was the major fatty
acid in leaves at two habitats, while linoleic acid was the major component in stem at
two habitats. Concerning the metabolic products studies, it was found that the percentage
of total nitrogen, total protein, total lipid and total, soluble and insoiuble carbohydrates
were higher in the leaves than those in the stems at both studied habitats, while they
were higher at Catherin than those at Wadi Fieran habitats. Concerning the active constituents it was found that the percentages of total
flavonoids, total alkaloids, total saponins and total tannins were higher at the leaves
than those in the stem, while they were higher at Calherin than those at Wadi Fieran
habitats.