At Sakha Agricultural Research Station, Cotton Research Institute, Agricultural Research Center, Egypt, six cotton varieties belong to (Gossypium barbadense L.) growing seasons 2020 and 2021. The F₁ hybrids and six cotton varieties Giza 96, Giza94, Giza 92(Egyptian cotton) and Australy13, Karshenky and Pima S₇ (anothervarieties) were growing in 2021 season in a randomized complete blocks design (RCBD) with three replications. Obtained results showed that the mean squares of parents and crosses were highly significant for all traits under study. The mean squares due to general combining ability (GCA) and specific combining ability(SCA) were highly significant for all traits under study except lint percentage at specific combining ability. The crosses Giza 96 × Karshenky, Giza94 × Australy13 and Australy13 × Karshenky were significant desirable heterosis relative to mid and better-parent for most yield traits under study, while, the crosses Giza 96 x Pima S7 and Giza 92 × Karshenky were significant desirable heterosis relative to mid and better-parent for most fiber traits under study. Highest broad-sense heritability estimates was observed in case of seed cotton yield plant^-1 with value of 96.81% and the lowest value was for lint percentage with value of 54.26%, while, narrow-sense heritability (h²_n) was ranged from 5.62% for micronare reading to 58.17% for lint cotton yield/plant, respectively.In general, Australy 13 and Karshenky can be used in breeding programs for improving high yielding varieties, while Giza 92 and Giza 96 can be considered as excellent parents for the production of new varieties characterized with best fiber properties.