This work aimed to study the effect of medium type, explant part and kinitin
concentration on in vitro multiplication as well as in vitro rooting of Eucalyptus grandis
to obtain the optimum regeneration phase and to develop the propagation method of
E, grandis The results indicated that, the highest number of formed shoots was
regarded with culturing the basal node on MS medium supplemented with 2mg/litre
kin. as compared with WPM medium. All treatments (is MS medium supplemented with ISA) induced root
formation on E. grandis shoots, but ISA at 2mgllitre recorded the best results. The
rooted shootlets (plantlets) were removed from rooting medium after seven weeks and
cultured in plastic pots 10 cm. diameter, containing peatmoos, clay and sand (1:1:1 by
volume) under greenhouse conditions for four weeks. The survival percentage of E. gandis plants recorded 83% . from the above
results. the micropropagation techniques strongly recommended to increase the
number of E. grandis plants in Egypt.