This study was conducted to explore the growth behavior of plants tissue culture  in vitro using an optical spectrum system exchange. In this investigation, the 3^rd generation in vitro plantlets of potato cv. "Diamond" were employed. The experiment included 5 treatments. The adventitious shoots of S. tuberosum (0.5–1 cm in length) were exposed in jars containing approximately 30 ml of the Murashige and Skoog (MS ) salt medium. The cultures were kept under following LED lights: White, Yellow, Green, Red and Blue respectively for 4 days switch within every light, compared to continuous white light for the same period (20 days). Comparing to the white light every 20 days found that the third subculture was significantly higher than the second and the first, in most optimal spectrum LED treatment color, also most averages of growth treatment was higher under red and blue LED light. the contents of amino acids and proteins exhibited accumulation trend with variant degree depend on type of light used. Generally, the combined light had the highest accumulation of amino acids and proteins compared to monochromatic light. For monochromatic light, the blue light recorded the most accumulation of amino acids and proteins compared to other lights. Also, plantlets exposed to red and blue light had the maximum levels of pigments. High accumulation of α─tocopherol content in the order, blue>red>green>yellow>white. Blue light and plants subjected to multiple light cycles were found to have the most accumulation in anthocyanin.