This study performed, al Central Laboratory for Research and Development 0
Date palm at Giza during the period from 2000-2002, to produce a re« date palm plant
instead 01 oldest. diseased, arrected by red (Incian) palm weevil trees and protect a
important seml-dry date palm culIivars from extinction, using tissue aJlture techniques Rapid production with maintain the original o"·shoots 01 some semi-dry date cuttiva (Amry and AgJany} in Sharlcia GowmoTBIe was the main tal"9Sl of this study. The results of this inwstigation the nghesl showed significant values of brownin~ percentage observed in the lear primordial explants of date palm Arnry CII. extended ;, th~ subsequent cultures for 32 week!. Callus formation was superior by using Amry CII .• shoot Up explanta and cuftuing explanls for 32 weeks. Embryogenic callus forma~on significanl1 affected by using different growth regulalM onlainng.medIa. MB medium produced th~ highest sg,ificant value of embfyogenlc c:allus formation and also Amry CII., culture perl~ for 32 weeks were superior inthisrespect.Embryogenic callus of Amry Cot, produced the highest significant value of embryo formation after 12 weeks. M1, M3 and M7 were the most etredive media to produce highest significant value of embryo formatiOn.